PEAKS Studio 4.5 - De Novo Sequencing Software

Waterloo, Canada - July 10, 2007…BSI (Bioinformatics Solutions Inc.) announced today, their much anticipated release of PEAKS 4.5 for peptide mass spectrometry analysis. PEAKS, known for its leadership role in de novo sequencing (sequencing of peptides not present in any database) is once again guiding tandem mass spectrometry researchers into in a new era of data analysis through sequence reconstruction. Accurate sequence reconstruction is performed by the built in SPIDER tool, which has been demonstrated to increase peptide findings by upwards of approximately 20%.

Other cutting-edge enhancements to PEAKS help researchers to identify peptides using predicted retention time. PEAKS provides all of the necessary tools for superior peptide sequence derivation and protein identification from tandem MS/MS spectra - all within an intuitive graphical interface. PEAKS Studio 4.5 provides fast, accurate results for spectra from both sequenced and unsequenced organisms. Instrument Capabilities: Researchers are now fully capable of processing raw data, straight from their instruments (Agilent, Bruker, Shimadzu, Thermo and Waters to name a few).

Here is what BSI has bundled into PEAKS 4.5:

• 10% - 30% improvement in de novo sequencing accuracy on ion-trap data.
• Improvements to the inChorus search engine:
  • Better integration with Sequest and Mascot - searches can be launched from inChorus, and/or existing results imported.
  • Existing results from any search engine can be loaded in (including PEAKS), avoiding having to repeat the search.
  • A separate local database is defined for recompiling the results based on peptides from all search engines.
  • The new RSD metric is available for all results, regardless of search engine.
• Improvements to instrument flexibility
  • Any combination of mass analyzer, fragmentation (incl. ETD) and ion source can be custom configured.
  • For ease of use, PEAKS will automatically check the data to detect the scan mode.
• A new, revolutionary tool in SPIDER allows you to not only find homologous peptides, but also reconstruct the real sequence, confirming mutation and correcting de novo sequencing errors.
• Retention time prediction: an RT is predicted for each peptide, and you can filter search results by (realRT – predictedRT).
• Data quality score now available, allowing a second evaluation of sequencing results trustworthyness.
• All parameters (start to finish) plus user’s notes included on result export.
• Improved charge and mono-isotopic peak picking mean even better coverage.
• Spectra can be exported to SVG, a standard graphics format, so you can move and resize parts of the picture.

Please contact Advanced Labs for a demo or a quotation.

Tags: peptides, protein, sequencing