FLIM/FRET Instruments

FLIM is the abbreviation for “Fluorescence Lifetime Imaging Microscopy”. It is a technique that maps the spatial distribution of lifetimes within microscopic images of fixed as well as of living cells. The fluorescence lifetime is the signature of a fluorescent material; it is the exponential decay in emission after the excitation. In other words, the lifetime is the average time that fluorescent molecules spend in the excited state.

FLIM can be measured in two ways: by the time domain method that is mainly applied on confocal laser scanning microscopes and by the frequency domain method that is used on widefield fluorescence microscopes.

LIFA - Lambert Instruments Fluorescence Attachment for lifetime imaging microscopy can be attached to any widefield fluorescence microscope and works in the frequency domain, which means that it uses a modulated light source and a modulated image intensifier as detector. The system is easy to install (within 1 hour) and to operate and does not require an optical bench with laser. The LIFA system has been judged “easy and highly quantitative”. And last but not least, it’s reasonably priced.Download PDF

Read Lambert FLIM/FRET News here.

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